If is concluded that embryonic stem cells can differentiate into hematopoietic cells and endothelial cells in combinant culture system of this study. On the 8(th) day of induction, all of the above genes except Zeta-globin were expressed, while ES cell and EB cells which served as controls did not express scl and Zeta-globin genes (4) on the 8(th) day of induction, the proportions of flk-1(+) cells and CD34(+) cells among all the inducing population were 9.8% and 16.8%, respectively, while the corresponding positive populations were 0.36% and 1.16% in spontaneously differentiated 11(th) day's EB, and 0.04% and 0.16%, respectively, in ES cells. In addition, CD45 positive in big cell colonies were also found in the semisolid medium (2) attached cell colonies appeared after culture for 8 days in the semisolid medium and VIII factor, UEA and KDR could be detected as negative by immunocytochemical staining (3) on the 4(th) day of induction, mRNAs of flk-1, BMI-1, scl and Zeta-globin were all expressed. The results demonstrated that: (1) cell clusters containing 20-30 cells were formed after culture for 8 - 14 days in the semisolid medium, replanting these cells resulted in similar cell cluster forming. Surface markers, such as flk-1, CD34 were tested by the flow cytometry. During the induction, mRNA expression of flk-1, BMI-1, scl, and Zeta-globin genes was tested by RT-PCR. Immunocytochemical staining was used to check the surface markers of the colony cells. Then, the differentiated cells were cultured in the semisolid medium containing 0.9% methylcellulose and cytokines to study the colony forming and self-renewal ability of cells. After culture for 3 days, EB cells were trypsinized into single cells and induced for 8 days by sccm + cys. To investigate the effect of stromal cell conditioned medium combined with cytokines (sccm + cys) on the differentiation from human embryonic stem cells to hematopoietic cells and endothelial cells, the mouse fibroblast feeder cells to make human embryonic stem cells grown into embryonic bodies (EBs) were initially deleted. What do you guys think? Is this doable? The 9999 ebuild shouldn't be put into the tree since it grabs from MakeHuman's server, and if hundreds of people use it, it'll bog down their server.Embryonic stem cells are pluripotent and their differentiation in vitro can serve as an experimental model to explore the molecular mechanisms of early embryonic development. The tarball can then be hosted on the developer's server. Then run 'makehuman-packager.sh X.X.X' to generate an ebuild, tarball, and Manifest. So basically, the process is, 'emerge =media-gfx/makehuman-9999' to install the latest development version. In the files directory, you can call './makehuman-packager.sh 1.0.0' to create a tarball of the MakeHuman program, and the generated tarball can be stored on Gentoo's mirror. This isn't an ideal way of doing, but this is where the meta-ebuild comes in. Makehuman-9999.ebuild retrieves the latest assets from the MakeHuman development FTP server in the src_install function, and installs. It was there to build the makehuman-1.0.0 ebuild. The makehuman-1.0.0-r1.ebuild should be ignored. I have created some ebuilds for MakeHuman if anyone wants to take a look at them. The MakeHuman 0.9.1 Release Candidate was published in December 2007, prompting considerable community feedback. In the xf86-video-i810 drivers which dramatically slows down OpenGLĬompiles, installs and loads perfectly well. add PDF documentation found on the web site for Makehuman, otherwise there'sįully tested on ~amd64 (without problem)and partially on x86 because of a bug Any help on that subject, among others, will be welcome. The true version is makehuman-0.9.1-rc1a, not I had to deal with makehuman version numerotation which does not matches the required standards. the makehuman icon does not appear in the menus. I was not able to easily use eautoreconf to regenarte autotools files in makehuman nor in mhgui without breaking linking with Freeglut. This are my first ebuilds, so please be indulgent to the remaining bugs or Please note that in the future releases, makehuman might also be able to use The current version is missing a few features as this is an RC1 alpha release.Ĭomparating to previous ebuilds makehuman-0.9.1.ebuild requires makehuman: Version: 0.9.1: Release: 0.9.rc1a.fc18: Epoch: Summary: Modeling of three-dimensional humanoid characters: Description: MakeHuman(C) is an open-source, open graphics library, stand-alone, multi-platform, software program, which is completely written in C++ language. Makehuman is a graphical tool for drawing and posing 3D humanoids. Please find attached makehuman-0.9.1.ebuild, animorph-0.3.ebuild and Makehuman-0.9.1.ebuild updated and renamed
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